AIMTo evaluate the feasibility and accuracy of continuous monitoring of drug with a fiber optic chemical sensor (FOCS) in animal.

METHODSAn accurate optical design was used to enhance the intensity of light from a 100-micron optic fiber and the fluorescence signal could be detected. A new sol-gel method was used to fix the fluorescence substance 4-(N, N-dioctyl) amino-7-nitrobenz-2-oxa-1, 3-diazole (D-70) on the body fiber. The vary quenching means the vary of the concentration of adriamycin (ADM) in rabbit blood. ADM was determined by FOCS based on the fluorescence multiple quenching. In a simple animal model, the carotid artery was catheterized with a cannula, housing a 100-micron optic fiber.

RESULTSThe recovery of ADM was 99.4%-106.2%, the within-run and between-run RSDs were 6.6%-11.4% and 5.9%-11.7% respectively. The method permitted detection limits as low as 0.057 microgram.mL-1 at a signal-to-noise ratio of 3.

CONCLUSIONFiber-optic chemical sensor is potentially useful for monitoring blood drug in biomedical field.