Effects of potassium channel blockers on the proliferation of rat bronchial smooth muscle cells.
- Author:
Xian-sheng LIU
1
;
Yong-jian XU
;
Zhen-xiang ZHANG
;
Wang NI
Author Information
- Publication Type:Journal Article
- MeSH: 4-Aminopyridine; pharmacology; Animals; Bronchi; cytology; Calcium; metabolism; Cell Division; drug effects; Cells, Cultured; Glyburide; pharmacology; Muscle, Smooth; drug effects; metabolism; Potassium Channel Blockers; pharmacology; Potassium Channels, Calcium-Activated; antagonists & inhibitors; Potassium Channels, Voltage-Gated; antagonists & inhibitors; Proliferating Cell Nuclear Antigen; metabolism; Rats; Rats, Sprague-Dawley; Tetraethylammonium; pharmacology
- From: Acta Pharmaceutica Sinica 2003;38(5):333-336
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effects of blockers of the three kinds of potassium channels: voltage-dependent K+ channel(KV), calcium-activated K+ channel(KCa) and ATP-sensitive K+ channel(KATP), on the proliferation of rat bronchial smooth muscle cells (BSMCs).
METHODSThe effects of three kinds of potassium channel blockers on the proliferation of BSMCs were detected by MTT method, immunocytochemistry staining and flow-cytometry. Their effects on the dynamic changes of Ca2+ concentration in BSMCs were investigated by the fluorophotometry of Fura-2/AM.
RESULTSThe KV blocker 4-aminopyridine (4-AP) was shown to significantly increase the expression of proliferating cell nucleus antigen in cultured rat BSMCs (P < 0.01), but the KCa blocker tetraethylammonium (TEA) and KATP blocker glibenclamide(Glib) did not show such effect (P > 0.05). 4-AP was found to significantly increase the optical density value of the cultured BSMCs (P < 0.01) by MTT method and the numbers of S + G2M BSMCs(P < 0.05) by flow-cytometry. TEA and Glib did not show such effects. 4-AP significantly increased the Ca2+ concentration in cultured BSMCs(P < 0.01). TEA and Glib did not show such effects.
CONCLUSIONThis result suggests that inhibition of KV activity can increase intracellular Ca2+ and proliferation of rat BSMCs, but inhibition of KCa and KATP showed no effect.
