Determination of tanshinone IIA in rat plasma and the pharmacokinetics by RP-HPLC method.
- Author:
Jin-ping QIAO
1
;
Pei-ling HOU
;
Ya-wei LI
;
Zeper ABLIZ
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anti-Infective Agents; blood; pharmacokinetics; Area Under Curve; Chromatography, High Pressure Liquid; methods; Diterpenes, Abietane; Drug Stability; Male; Phenanthrenes; blood; pharmacokinetics; Random Allocation; Rats; Rats, Sprague-Dawley
- From: Acta Pharmaceutica Sinica 2003;38(5):368-370
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo develop a sensitive and rapid HPLC method for the determination of tanshinone IIA (TS) in rat plasma and to study its pharmacokinetics in rats.
METHODSTS and 4-chlorodiphenyl (internal standard) were extracted from plasma with ethyl acetate. After liquid-liquid extraction, the sample was analyzed by HPLC with YMC C18 column (5 microns, 150 mm x 3.0 mm ID). The mobile phase consisted of acetontrile-water-acetic acid (74:26:1) at the flow rate of 0.3 mL.min-1, the UV detection wave length was 270 nm.
RESULTSThe calibration curve was linear (r = 0.9981) in the range from 0.05 to 6.40 mg.L-1. The lowest detectable concentration was 0.05 mg.L-1. The recoveries at the concentration of 0.05, 1.60 and 6.40 mg.L-1 were 98.9%, 102.1% and 100.4%, respectively. The inter- and intra-day RSDs were all less than 5%.
CONCLUSIONThis method is proved to be rapid, precise and reliable enough to be applied to the pharmacokinetics studies of TS in rats after a single dose of 15 mg.kg-1 by oral administration.
