Effects of human recombinant interleukin-1 receptor antagonist on isolated trachea smooth muscle of the guinea pig.
- Author:
Xiao-qun GE
1
;
Ji-ping LI
;
Hong-quan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Female; Guinea Pigs; In Vitro Techniques; Interleukin 1 Receptor Antagonist Protein; Male; Muscle Contraction; drug effects; Muscle, Smooth; drug effects; Receptors, Interleukin-1; antagonists & inhibitors; Recombinant Proteins; pharmacology; Sialoglycoproteins; pharmacology; Trachea; cytology
- From: Acta Pharmaceutica Sinica 2003;38(6):409-411
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the effects of human recombinant interleukin-1 receptor antagonist (IL-1ra) on isolated trachea smooth muscle (TSM) of the guinea pig.
METHODSChanges of the tension of isolated trachea were measured by force-displacement transducer and MedLab recording system in vitro.
RESULTSIL-1ra showed direct relaxed effect on TSM in normal and ovalbumin sensitized guinea pig. The EC50 values were 8.06 x 10(-8) and 5.88 x 10(-7) mol.L-1 respectively. IL-1ra (1 x 10(-7)-1 x 10(-5) mol.L-1) concentration-dependently inhibited the contraction of TSM induced by 1 x 10(-3) mol.L-1 histamine (His), 1 x 10(-3) mol.L-1 acetylcholine (ACh) and 1 x 10(-6) mol.L-1 5-hydroxytryptamine (5-HT) (P < 0.05 or 0.01). When IL-1ra was given in advance, the contractile actions of His, ACh and 5-HT were antagonized by IL-1ra (1 x 10(-7)-1 x 10(-5) mol.L-1), the pD'2 value for His was 6.6 +/- 0.3. However, the contractile action of ACh was enhanced by IL-1ra at low concentration of 1 x 10(-9)-1 x 10(-8) mol.L-1. IL-1ra significantly prevented and inhibited the contraction of sensitized TSM induced by antigen ovalbumin, the IC50 value was 4.48 x 10(-7) mol.L-1.
CONCLUSIONThe results indicate that IL-1ra, within certain concentration, can relax the normal, contracted and sensitized ISM of the guinea pig in vitro.
