Pharmacokinetic study on spinosin in rat plasma after oral administration of suanzaoren extract at a single dose.
- Author:
Yu-juan LI
1
;
Xin-miao LIANG
;
Hong-bin XIAO
;
Kai-shun BI
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Oral; Animals; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; administration & dosage; isolation & purification; pharmacokinetics; Flavonoids; blood; isolation & purification; pharmacokinetics; Plants, Medicinal; chemistry; Rats; Rats, Wistar; Seeds; chemistry; Ziziphus; chemistry
- From: Acta Pharmaceutica Sinica 2003;38(6):448-450
- CountryChina
- Language:English
-
Abstract:
AIMTo study the pharmacokinetics of spinosin in rat plasma after oral administration of Suanzaoren extract using sulfamethoxazole (SMZ) as internal standard by RP-HPLC method.
METHODSPlasma samples were deproteined with acetonitrile, followed by evaporation of the acetonitrile to dryness. The residual was then resolved in mobile phase and HPLC separation was achieved on a Hypersil C18 (5 microns, 200 mm x 4.6 mm ID) column at 35 degrees C. The mobile phase consisted of acetonitrile-water-acetic acid (15:85:1) at a flow rate of 0.7 mL.min-1. The UV detection wavelength was set at 334 nm.
RESULTSThe calibration curve was shown to be linear over the range from 18.1 to 903.5 micrograms.L-1 (r2 > or = 0.995). Mean recovery was 94.5%. Within-day and between-day precisions RSD were less than 9.0%. The limit of quantitation was 18.1 micrograms.L-1. The plasma spinosin was stable at -20 degrees C.
CONCLUSIONThe simple, sensitive and accurate HPLC method developed has been applied to determine the pharmacokinetics of spinosin in rat plasma after having taken Suanzaoren extract at a single dose.
