Pharmacokinetics of the alkaloids from the processed seeds of Strychnos nux-vomica in rats.
- Author:
Xiao-yue XU
1
;
Bao-chang CAI
;
Yang PAN
;
Tian-shan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; isolation & purification; pharmacokinetics; Animals; Cyclic N-Oxides; isolation & purification; pharmacokinetics; Drugs, Chinese Herbal; isolation & purification; pharmacokinetics; Female; Hot Temperature; Male; Plants, Medicinal; chemistry; Rats; Rats, Sprague-Dawley; Seeds; chemistry; Strychnine; analogs & derivatives; isolation & purification; pharmacokinetics; Strychnos nux-vomica; chemistry; Technology, Pharmaceutical; methods
- From: Acta Pharmaceutica Sinica 2003;38(6):458-461
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the pharmacokinetic process about the concentration in rat plasma of the alkaloids from processed seeds of Strychnos nux-vomica with RP-HPLC method.
METHODSHypersil BDS C18 column was used and the mobile phase consisted of acetonitrile-water at the flow rate of 0.8 mL.min-1. The UV detection wave length was 254 nm.
RESULTSThe concentration-time data of strychnine, brucine, strychnine N-oxide and brucine N-oxide were all in accordance with an open two-compartment model after i.v. alkaloids. Their parameters were as follows: T1/2 alpha were (8 +/- 5), (4 +/- 3), (6.2 +/- 1.7) and (3.0 +/- 0.8) min, T1/2 beta were (262 +/- 125), (416 +/- 131), (285 +/- 50) and (342 +/- 141) min, CL were (17 +/- 4), (21 +/- 12), (1.9 +/- 1.8) and (2.8 +/- 1.1) mL.min-1, Vc were (1.4 +/- 0.5), (1.7 +/- 1.1), (0.24 +/- 0.16) and (0.23 +/- 0.06) L.kg-1, Vd were (6.0 +/- 1.2), (12 +/- 7), (0.8 +/- 0.6) and (1.5 +/- 0.6) L.kg-1, AUC were (57,578 +/- 25,578), (35,240 +/- 15,616), (93,088 +/- 22,375) and (177,712 +/- 120,110) h.microgram.L-1, respectively.
CONCLUSIONThe method is a good reference for pharmacokinetics in human bodies.
