Degradation of electrodeposited calcium phosphate and calcium phosphate-chitosan coatings in vitro

VernacularTitle:电化学沉积钙磷涂层和钙磷-壳聚糖涂层的体外降解实验
Author: Li-Na WANG ¹ ; Yi-Ning WANG ; Jia-Wei WANG
Author Information

1. 武汉大学口腔医学院
Keywords: Osteoclasts; Electrochemistry; Calcium phosphate coating; Chitosan
From: Chinese Journal of Stomatology 2008;43(6):374-375
CountryChina
Language:Chinese
Abstract: Objective To investigate the degradation of electrodeposited calcium phosphate (ECP) coating and calcium phosphate/chitosan(ECPC) coating in vitro. Methods Osteoclasts were isolated from neonatal rabbit long bone cavities and incubated with ECP and ECPC coatings. Calcium ion concentrations in the culture medium were analyzed at 3 days and 6 days. The osteoclastic resorption was observed with scanning electron microscope. Results Both coatings demonstrated osteoclastic resorption lacunae. The calcium ion concentrations of the culture mediums were decreased when incubated with calcium phosphate coatings (P < 0.05 ). Compared with coatings cultured with osteoclasts, the calcium ion concentrations of those cultured without osteoclasts were higher on day 3 (P > 0. 05 ) but lower on day 6 ( P < 0. 05 ).Conclusions Both ECP and ECPC coatings can be resorpted by osteoclasts in vitro and can dissolve in the culture medium.