Effect of Escherichia coli lipopolysaccharide on mineralized matrix formation in vitro differentiation human dental pulp cell.
- Author:
Hong-wei JIANG
1
;
Jun-qi LING
;
Jin-feng ZENG
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; metabolism; Cell Differentiation; drug effects; Cells, Cultured; Dental Pulp; cytology; Escherichia coli; Humans; Lipopolysaccharides; pharmacology; Minerals; metabolism; Odontoblasts; drug effects; Osteocalcin; metabolism
- From: Chinese Journal of Stomatology 2008;43(7):429-430
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigated the effect of Escherichia coli (Ec) LPS on alkaline phosphatase (ALP) activity and expression of dentin sialophosphoprotein (DSPP) and osteocalcin (OCN) genes in vitro differentiation human dental pulp cell.
METHODSOdontoblast-like cells were cultured, cells exposed to Ec LPS for 12 h, total RNA was isolated and DSPP, OCN transcripts were examined by real-time RT-PCR. ALP kit were used to assessed the changes of ALP activity.
RESULTSReal-time RT-PCR analysis indicated that Ec LPS induced about a 3.6-fold decrease for DSPP gene and a 1.6-fold decrease for OCN gene in odontoblast-like cells as compared with controls. At the same time, cells treated with LPS could depress ALP activity from (1156.10 +/- 100.60) pmol x h(-1) x ng(-1) down to (884.80 +/- 26.72) pmol x h(-1) x ng(-1).
CONCLUSIONSThese results indicate that exposure of odontoblast-like cells to LPS can alter cells function by downregulating cell markers of odontoblastic activity.
