Effect of Escherichia coli lipopolysaccaride on mineralized matrix formation in vitro differentiation human dental pulp cell

VernacularTitle:脂多糖对诱导分化的牙髓细胞形成矿化基质的影响
Author: Hong-Wei JIANG ¹ ; Jun-Qi LING ; Jin-Feng ZENG
Author Information

1. 中山大学光华口腔医学院
Keywords: Dental pulp; Lipopolysaccarides; Mineralized matrix
From: Chinese Journal of Stomatology 2008;43(7):429-430
CountryChina
Language:Chinese
Abstract: Objective To investigated the effect of Escheerichia coli(Ee) LPS on alkaline phosphatase(ALP) activity and expression of dentin sialophosphoprotein (DSPP) and osteocalcin (OCN) genes in vitro differentiation human dental pulp cell. Methods Odontoblast-like cells were cultureed, cells exposed to Ec LPS for 12 h ,total RNA was isolated and DSPP、OCN transcripts were examined by real-time RT-PCR. ALP kit were used to assessed the changes of ALP activity. Results Real-time RT-PCR analysis indicated that Ec LPS induced about a 3.6-fold decrease for DSPP gene and a 1.6-fold decrease for OCN gene in odontoblast-like cells as compared with controls. At the same time, cells treated with LPS could depress ALP activity from (1156.10±100.60)pmol·h-1·ng-1 down to (884.80±26.72)pmol·h-1·ng-1. Conclusions These results indicate that exposure of odontoblast-like cells to LPS can alter cells function by downregulateing cell markers of odontoblastic activity.