Regulation of collagen type Ⅰ and fibronectin mRNA expression by mechanical stress in cultured human periodontal ligament fibroblasts
10.3321/j.issn:1002-0098.2008.07.016
- VernacularTitle:应力对人牙周膜成纤维细胞Ⅰ型胶原和纤连蛋白mRNA表达的影响
- Author:
Qing-Dang ZHU
1
;
Yong-Lie CHAO
;
Xin-Min CHEN
;
Jun HU
Author Information
1. 浙江大学医学院附属口腔医院
- Keywords:
Fibroblasts;
Stress,mechanical;
Collagen type Ⅰ;
Fibronectins
- From:
Chinese Journal of Stomatology
2008;43(7):434-436
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of different dynamic tensional and compressive stress on the mRNA expression of collagen type Ⅰ and fibronectin in cultured human periodontal ligament fibroblasts (hPDLF), and explore the regularity of functional change in hPDLF. Methods A new cyclic strain loading apparatus was used for mechanically loading, Cells cultured in vitro were loaded with three levels (1000 μstrain, 2000 μstrain, 4000 μstrain) of tensional and compressive forces and collected at different time (0 h,0. 5 h,1 h,4 h,8 h,12 h) course after strain loading. The quantity of collagen type Ⅰ and fibronectin mRNA was analyzed by means of quantitative real-time PCR with special primers of up- and down-regulated genes. Data were analyzed using SPSS version 10. 0 software. Results Different magnitude and different kinds of mechanical forces as well as the force application time significantly changed the expression of collagen type Ⅰ and fibronectin mRNA in hPDLF. Conclusions Dynamic mechanical forces could regulate the expression of collagen type Ⅰ and fibronectin mRNA in hPDLF. Collagen type Ⅰ and fibronectin participated in the mechanical signal transduction in human periodontal ligament fibmblasts.
