Preparing of semiconductor quantum dots-Smad4 monoclonal antibody fluorescent probes and identification of their ability of specific immuno-recognition
10.3321/j.issn:1002-0098.2008.08.012
- VernacularTitle:半导体量子点-Smad4单克隆抗体荧光探针的制备及免疫特异识别能力的检测
- Author:
De-Ping SUN
1
;
Kai YANG
;
Rui CHEN
Author Information
1. 重庆医科大学附属第一医院
- Keywords:
Quantum dots;
Molecular probes;
Fluoroimmunoassay
- From:
Chinese Journal of Stomatology
2008;43(8):492-494
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare semiconductor quantum dots (SQD)-Smad4 monoclonal antibedyfluorescent probes and to detect the optical qualities and the ability of specific recognition of the probes. Methods SQD were chemically modified with Smad4 monoclonal antibody proteins to prepare water soluble probes, and the fluorescence intensity, photostability, absorption spectra and emission spectra of the probes were studied. The location of Smad4 in rat dental papillae cells (RDPC) was examined by SP anti-Smad4 immunocytochemical method and SQD-Smad4 direct immunofluorescent imaging. Results SQD and monoclonal antibody covalently bonded to form the fluorescent probes which could specifically recognize Smad4 in RDPC. These fluorescent probes still had properties, including broad absorption band, narrow emission band, high fluorescence intensity and photostability. Conclusions SQD and monoclonal antibody could eovalently bond to form the fluorescent probes with distinct optics character and ability of specificrecognization, which provides the scientific evidence that SQD trace the molecular movement in living cells inlong-term, in situ and in real time.
