Multilineage differentiation of human dental pulp cells and periodontal ligament cells in vitro
10.3321/j.issn:1002-0098.2008.08.013
- VernacularTitle:人牙髓细胞与牙周韧带细胞多向分化能力的比较
- Author:
Xi WEI
1
;
Li-Ping WU
;
Jun-Qi LING
;
Lu LIU
Author Information
1. 中山大学
- Keywords:
Cell differentiation;
Mesenchymal stem cells;
Dental pulp cells;
Periodontalligament cells
- From:
Chinese Journal of Stomatology
2008;43(8):495-499
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the multilineage differentiation potential of human dental pulp cells (DPC) and periodontal ligament cells (PDLC) in vitro, and to identify the stem cell characteristics. Methods Human DPC and PDLC were isolated by enzymatic digestion. STRO-1 expression was investigated by flow cytometry. Cells were induced to odontogenic/osteogenic differentiation, adipogenic differentiation and chondrogenic differentiation. The multilineage differentiation capacities of DPC and PDLC were evaluated by yon Kossa stain, anti-osteocalcin (OCN) and anti-dentin sialoprotein (DSP) immunocytochemistry, oil red O stain, Alcian blue stain, anti-collagen type Ⅱ immunocytochemistry, and real time RT-PCR. Results Colony formation was observed in DPC and PDLC, with STRO-1 positive rate of ( 16. 5% ± 4. 2% ) and ( 11.6% ± 1.1% ) respectively. Multilineage differentiation was demonstrated in 100% of DPC samples in contrast to 83.3% of PDLC samples. OCN, dentinsialophosphoprotein ( DSPP),peroxisome-proliferator-activated receptor gamma 2 (PPARγ2), lipoprotein lipase (LPL) and collagen type Ⅱ mRNA levels in DPC and PDLC were significantly upregulated after induction (P <0. 001 ). There were significant differences in the ratio of upregulation of OCN and PPARγ2 mRNAs between DPC and PDLC (P <0. 001 ). Conclusions DPC and PDLC contain similar proportion of mesenchymal stem cells and possess comparable multilineage differentiation capacities.
