Expression of growth factor, extracellular matrix and antioxidant (N-acetylcysteine) effect in TGF beta1 treated rat lens system.
- Author:
Min Soo PARK
1
;
Chung Sook AHN
;
Young Sook HONG
Author Information
1. Gong Eye Medical Center, Korea. meanpark@hanmail.net
- Publication Type:Original Article
- Keywords:
Lens epithelial cell;
mRNA;
Growth factors;
Antioxidants
- MeSH:
Actins;
Animals;
Antioxidants;
Blotting, Western;
Cataract;
Collagen Type I;
Connective Tissue Growth Factor;
Cysteine;
Epidermal Growth Factor;
Epithelial Cells;
Extracellular Matrix Proteins;
Extracellular Matrix*;
Fibroblast Growth Factors;
Fibronectins;
Intercellular Signaling Peptides and Proteins;
Rats*;
Receptor, Epidermal Growth Factor;
Receptors, Fibroblast Growth Factor;
Receptors, Growth Factor;
Receptors, Transforming Growth Factor beta;
RNA, Messenger;
Transforming Growth Factor beta
- From:Journal of the Korean Ophthalmological Society
2002;43(12):2543-2554
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The objective of this study was to characterize transdifferentiated lens epithelial cells analyzed by reverse transcription-polymerase chain reaction (RT-PCR) for the expression of mRNAs encoding growth factors, growth factor receptors and pathologic extracellular matrix proteins and by Western blot analysis for the proteins encoded by these mRNAs. Moreover, after antioxidants treatment, such as Nacetyl cysteine (NAC), we observed the effect on changes in the expression of growth factors, growth factor receptors and extracellular matrix proteins. METHODS: TGF-beta treated rat lens cultured with medium 199 (Sigma Co. St. Louis, MO) was subject to RT-PCR and Western blot analysis to assess expression of mRNAs and proteins encoded by these mRNAs. RESULTS: The expression of mRNAs for TGF-beta 1, TGF-beta 2, TGF-beta 3, TGF-beta receptor, epidermal growth factor (EGF), epidermal growth factor receptor, fibroblast growth factor (FGF), fibroblast growth factor receptor and connective tissue growth factor (CTGF) were increased. The levels of type I collagen, fibronectin, and alpha-smooth muscle actin (SMA) mRNAs were also increased. However, the expression of growth factors, receptors, extracellular matrix were decreased by antioxidant, such as NAC. CONCLUSIONS: The enhanced expression of growth factors, growth factor receptors and extracellular matrix in present the molecular mechanism underlying pathogenesis of cataracts. And the suppression of growth factors and growth factor receptors with treatment of antioxidants, such as NAC, suggests the possibility of using drugs in the prevention or treatment of cataracts.
