Expression feature and bioinformatic analysis of molar root patterning gene 1.
- Author:
Xiang-hui XING
1
;
Xiao-jing WANG
;
Shi-bin YU
;
Kun XUAN
;
Yan JIN
;
Ling-ying WEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Computational Biology; Gene Expression Regulation, Developmental; Genes; Molar; growth & development; Molecular Sequence Data; Protein Sorting Signals; genetics; Proteins; genetics; metabolism; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; Tooth Root
- From: Chinese Journal of Stomatology 2008;43(9):528-530
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo examine the expression of molar root patterning gene 1 (Mrp1) and predict the Mrp1 structure by bioinformatics analysis.
METHODSA pair of Mrp1-specific PCR primers were designed, and RT-PCR method was used to study the mRNA's expression pattern in rat molar root and other organs. Gene positioning and other protein sequence prediction were carried out by chromosome analysis and other bioinformatics analysis.
RESULTSMrp1 was expressed not only in the molar but also in the developing pancreas, liver, lung and kidney tissues. Mrp1 was located in the 18q12.3 chromosome of the rats and the Mrp1 amino acids sequence had about 37% homology with a known protein Uroplakin IIIb (p35) which was an urothelial differentiation membrane molecular marker. A trans-membrane structure, 5 PKC phosphorylation sites and 4 CKII phosphorylation sites in Mrp1 were found.
CONCLUSIONSMrp1 has a broad expression in different developing organs, and it may have a important function in the rat tooth root development.
