Expression feature and bioinformatic analysis of molar root patterning gene 1

VernacularTitle:磨牙牙根发育模式基因1在大鼠多脏器中的表达及其结构分析
Author: Xiang-Hui XING ¹ ; Xiao-Jing WANG ; Shi-Bin YU ; Kun XUAN ; Yan JIN ; Ling-Ying WEN
Author Information

1. 第四军医大学口腔医学院
Keywords: Genes,developmental; Reverse transcriptase polymerase chain reaction; Computational biology; Molar root patterning gene 1
From: Chinese Journal of Stomatology 2008;43(9):528-530
CountryChina
Language:Chinese
Abstract: Objective To examine the expression of molar root patterning gene 1 (Mrp1) and predict the Mrp1 structure by bioinformatics analysis. Methods A pair of Mrp1-specific PCR primers were designed, and RT-PCR method was used to study the mRNA's expression pattern in rat molar root and other organs. Gene positioning and other protein sequence prediction were carried out by chromosome analysis and other bioinformaties analysis. Results Mrp1 was expressed not only in the molar but also in the developing pancreas, liver, lung and kidney tissues. Mrp1 was located in the 18q12.3 chromosome of the rats and the Mrpl amino acids sequence had about 37% homology with a known protein Uroplakin Ⅲb (p35) which was an urothelial differentiation membrane molecular marker. A trans-membrane structure, 5 PKC phosphorylation sites and 4 CK Ⅱ phosphorylation sites in Mrpl were found. Conclusions Mrpl has a broad expression in different developing organs, and it may have a important function in the rat tooth root development.