Expression and significance of cartilage oligomeric matrix protein, C-X-C motif 9 and keratin 19 in oral submucosal fibrosis
10.3321/j.issn:1002-0098.2008.09.011
- VernacularTitle:软骨寡聚基质蛋白、趋化因子9和细胞角蛋白19在口腔黏膜下纤维化中的表达
- Author:
Ning LI
1
;
Xin-Chun JIAN
Author Information
1. 中南大学湘雅医院
- Keywords:
Oral submucous fibrosis;
Immunohistochemistry;
Blotting,western;
Reverse transcriptase polymerase chain reaction
- From:
Chinese Journal of Stomatology
2008;43(9):551-555
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and localization of cartilage oligomeric matrix protein(COMP), C-X-C motif 9(CXCL9) and keratin 19(KRT19) in oral submucons fibrosis (OSF) and to evaluate their roles in the pathopoiesis of OSF. Methods The expression and localization of COMP,CXCL9 and KRT19 were investigated in the specimens of 66 patients with oral submucous fibrosis and 14 normal controls by immunohistochemistry, and their protein and mRNA expressions were detected by Western blotting and RT-PCR. Results COMP was overexpressed in 36 (55%) cases of OSF, and 43 (65%) cases showed positive immunoreactivity for CXCL9 protein in the cytoplasm of inflammatory cells and endothelial cells in OSF. All normal buccal mucosa tissues were stained continuously and strongly for KRT19 in the cytoplasm of basal cells, only 7 (11%) of 66 OSF samples showed faint and fragmented KRT19 staining in the cytoplasm of basal cells. RT-PCR and Western blotting results were fully consistent with the immunohistochemical data. Conclusions COMP, CXCL9 and KRT19 play an important role in the pathopoiesis of OSF.
