Antlangiogenic and antitumor effects of vascular endothelial growth factor small interfering RNA on Tea8113 human tongue sqnamous cell carcinoma xenografts in vivo
10.3321/j.issn:1002-0098.2008.09.012
- VernacularTitle:血管内皮生长因子小干扰RNA抑制人舌鳞状细胞癌细胞移植瘤生长及血管生成的实验
- Author:
Da-Hai YU
1
;
Jing LI
;
Ying CAO
;
Hai-Bo CHEN
;
Jie HAO
Author Information
1. 广西医科大学附属口腔医院
- Keywords:
Carcinoma,squamcoss cell;
Vascular endothelial growth factor;
Small interfering RNA
- From:
Chinese Journal of Stomatology
2008;43(9):556-560
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the antiangiogenic and antitumor effects of vector-based small interfering RNA(siRNA) targeting vascular endothelial growth factor(VEGF) on human tongue squamous cell carcinoma xenografts in vivo. Methods Tca8113 human tongue cancer nude mice xenograft model was established, and subsequently divided into four groups randomly (5/group). Two siRNA targeting VEGF constructed in enkaryotic expression vector (PU-VEGF-siRNA1, PU-VEGF-siRNA2) were injected introtumor and peritumor with lipofectamine 2000, respectively. No siRNA vector injected and non-injected tumors were used as experimented and negative controled,respetively. Animals were injected one time every 3 days for a total of 10 times. Three days after the last injection, the weigh and volume of tumors, and introtumor microvessel density (MVD) were measured. The expression of VEGF in xenngraft tumors was detected by reverse transcription pelymerse chain reaction (RT-PCR), Western blotting and immunohistochemistry. The cell apoptosis and cell cycle analysis of tumors were detected by Tunel and Flow cytometry, respectively. Results Compared to the experimental and negative controls, the percentage of cells in the G<,1> phase increased(P <0.05), the expression of VEGF on both mRNA and protein level, the tumor weigh and volume, and MVD decreased in the PU-VEGF-siRNA2 group (P<0.05), and more apoptesis was induced(P<0.01). But significant differences were not noted between PU-VEGF-siRNA1 group and two controls (P>0.05). Conclusions VEGF- siRNA can reduce VEGF expression, inhibit tumor growth and angiogenesis, and induce apoptosis in Tea8113 cell carcinoma in vivo. Different VEGF-siRNA may have different effect in vivo.
