Expression of chemokine receptor-7 in oral squamous cell carcinoma and its relation to cervical lymph node metastasis
10.3321/j.issn:1002-0098.2008.10.005
- VernacularTitle:口腔鳞状细胞癌中趋化因子受体CCR7的表达及其与颈部淋巴结转移的关系
- Author:
Zheng-Jun SHANG
1
;
Ke LIU
;
Xin-Ming CHEN
Author Information
1. 武汉大学口腔医学院
- Keywords:
Carcinoma,squamous cell;
Neoplasm metastasis;
Receptors,chemokine
- From:
Chinese Journal of Stomatology
2008;43(10):592-596
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of chemokine receptor CCR7 in oral squamous cell carcinoma (OSCC) and its possible role in directional neck lymph node metastasis. Methods The expression of chemokine receptor CCR7 was examined in 85 cases of OSCC,normal oral mucosa,and TcaS113 and adenoid cystic carcinoma (ACC-M) cell lines using immunohistochemistry,RT-PCR and Western blotting.The relationship between the expression and clinicopathological factors of OSCC was analyzed.After treatment with or without CCR7 antibody,in vitro adhesion assay was performed to compare the adhesion of Tea8113 and ACC-M cells to lymph nodes. Results CCR7 was expressed in 66% (56/85)of OSCC cases,and there was a significant difference in CCR7 expression between metastasis group (31/39,79%) and non-metastasis group (25/46,54%).RT-PCR and Western blotting also confirmed the presence of positive CCR7 expression in OSCC. Tea8113 cells expressing CCR7 showed stronger ability to adhere to lymph nodes as compared to CCR7-negative ACC-M cells and could be actively inhibited by CCR7 antibody.Conclusions CCR7 may play an important role in the development and lymph node metastasis of oral squamous cell carcinoma.
