Occurrence of supernumerary upper incisor teeth in Pax6-/- mouse fetuses
10.3321/j.issn:1002-0098.2008.11.010
- VernacularTitle:Pax6基因纯合突变胎鼠上前牙区额外牙发生的初步研究
- Author:
Hai-Hua LEI
1
;
He LIU
;
Xiang GAO
;
Jin-Hua WEN
;
Li-Hong GE
Author Information
1. 北京大学口腔医学院(口腔医院)
- Keywords:
Tooth,supernumerary;
Mutation;
Pax6 gene;
Mouse fetuses
- From:
Chinese Journal of Stomatology
2008;43(11):675-679
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the occurrence of supernumerary upper incisor teeth in Pax6-/- mouse fetuses and to provide a model to explore the role of Pax6 in the upper incisor development and the mechanism of supernumerary teeth involving Pax6. Methods Twenty Pax6-/- mouse fetuses of strain DEBA were isolated on E18.5 (embryonic day). The fetuses were sectioned serially in coronal plane and stained with haematoxylin and ernsin, then the presence of supernumerary teeth in the upper anterior area was examined histologically, and also the number, morphology and structure of lower incisor germs and the first and second molar germs in the maxilla and mandible were observed histologically. Eighteen E18.5 mouse fetuses of strain DEBA with Pax6 +/+ genotype were used as control. Results Of the 20 Pax6-/- fetuses examined, four possessed a single supernumerary tooth in the upper inciors' region. No supernumerary upper incisor teeth were observed in any of the 18 Pax6 +/+ fetuses examined. In the regions of lower incisors and the first and second molars of the maxilla and mandible, no significant difference was observed between Pax6-/- and Pax6 +/+ fetuses regarding the number, morphology and structure of tooth germs. Conclusions The results suggest that Pax6 played an important role in the development of upper incisor teeth in mice.
