Molecules of G2/M phase and the phosphorylation of survivin in the carcinogenesis of oral submucosal fibrosis
10.3321/j.issn:1002-0098.2008.12.002
- VernacularTitle:口腔黏膜下纤维化癌变过程中G2、M期细胞周期蛋白与存活素磷酸化的研究
- Author:
Shang-Hui ZHOU
1
;
Li-Li LI
;
Xin-Chun JIAN
;
Ying WANG
;
Xin-Qun CHEN
;
Xing GAO
Author Information
1. 中南大学湘雅医院
- Keywords:
Cell cycle;
Oral submucosa fibrosis;
Cyclin B
- From:
Chinese Journal of Stomatology
2008;43(12):709-712
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the expression of Cyclin B1,p34cdc2 and the phosphorylation of survivin(p-survivin)in oral squamous cell carcinoma and oral submucosa fibrosis(OSF),and to discuss their possible role in carcinogenesis of OSF.Methods The expression of Cyclin B1,p34cdc2 and p-survivin were analyzed by Western blotting assay in 10 cases of normal oral mucosa epithelium,40 cases of OSF epithelium and 42 cases of oral squamous cell carcinoma(OSCC)originated from OSF,respectively.Immunoprecipitation was used to confirrn the relationship between the p34cdc2 and survivin.Results The expression of Cyclin B1,p34cdc2,p-p34cdc2 and p-survivin in OSF group were significantly higher than those in normal group(P＜0.05).The expression of these molecules showed significant different(P＜0.05)between the OSF and OSCC originated from OSF,but there was no significant difference among the early stage,the moderately advanced stage and the advanced stage of OSF.Immunoprecipitation assay confirmed the combination of p34cdc2 and survivin.Conclusions The important molecules in G2/M phase-Cyclin B1,p34cdc2 and p-survivin may play a key role during the mitosis and proliferation of OSF,which will be helpful in early diagnosis and therapy of carcinogenesis of OSF.
