Effects of CYP2C19 Genetic Polymorphisms on PK/PD Responses of Omeprazole in Korean Healthy Volunteers.
10.3346/jkms.2017.32.5.729
- Author:
Sunny PARK
1
;
Yang Jin HYUN
;
Yu Ran KIM
;
Ju Hyun LEE
;
Sunae RYU
;
Jeong Mi KIM
;
Woo Yong OH
;
Han Sung NA
;
Jong Gu LEE
;
Doo Won SEO
;
In Yeong HWANG
;
Zewon PARK
;
In Jin JANG
;
Jaeseong OH
;
Seung Eun CHOI
Author Information
1. Clinical Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju, Korea. choi77@korea.kr
- Publication Type:Clinical Trial ; Original Article
- Keywords:
Omeprazole;
CYP2C19;
Genetic Polymorphisms
- MeSH:
Area Under Curve;
Chromatography, Liquid;
Cytochrome P-450 CYP2C19*;
Genotype;
Healthy Volunteers*;
Hydrogen-Ion Concentration;
Methods;
National Institutes of Health (U.S.);
Omeprazole*;
Phenotype;
Plasma;
Polymorphism, Genetic*;
Polymorphism, Single Nucleotide;
Tandem Mass Spectrometry;
Volunteers
- From:Journal of Korean Medical Science
2017;32(5):729-736
- CountryRepublic of Korea
- Language:English
-
Abstract:
The aim of this study was to examine the effects of CYP2C19*2 and *3 genetic polymorphisms on omeprazole pharmacokinetic (PK) and pharmacodynamic (PD) responses. Twenty-four healthy Korean volunteers were enrolled and given 20 mg omeprazole orally once daily for 8 days. The genotypes of CYP2C19 single nucleotide polymorphisms (SNPs) (*2, *3, and *17) were screened. The plasma concentrations of omeprazole, omeprazole sulfone, and 5-hydroxy (5-OH) omeprazole were determined by liquid chromatography with tandem mass spectrometry (LC-MS/MS). The noncompartmental method was used for the determination of PK parameters. Change of mean pH and proportion (%) of time of gastric pH above 4.0 were estimated. The poor metabolizer (PM) group had the lowest metabolic ratio and exhibited the highest area under the curve (AUC) for omeprazole among the CYP2C19 phenotype groups. The PM group showed the greatest change of mean pH and the highest % time of gastric pH above 4.0. The relationship between AUC of omeprazole and % time of gastric pH above 4.0 was confirmed. The study demonstrates that CYP2C19*2 and *3 influence the PKs and PDs of omeprazole in Korean healthy volunteers. Clinical trial registry at the U.S. National Institutes of Health (https://clinicaltrials.gov), number NCT02299687.
