Effect of Nitric Oxide on Adhesion and Migration of Trabecular Meshwork Cells.
10.3341/jkos.2013.54.4.639
- Author:
Jin Seong BAE
1
;
Jae Woo KIM
Author Information
1. Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Adhesion;
Migration;
Nitric oxide;
Trabecular meshwork cells
- MeSH:
Humans;
Nitric Oxide;
S-Nitroso-N-Acetylpenicillamine;
Stress, Mechanical;
Trabecular Meshwork
- From:Journal of the Korean Ophthalmological Society
2013;54(4):639-644
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the effect of nitric oxide (NO) on the adhesion and migration of cultured human trabecular meshwork cells (HTMC). METHODS: For adhesion assay, primarily cultured HTMC were attached to culture dishes for 1 hr, cells were rinsed, and the remaining adherent cells were assessed with MTT assay. Degree of cellular migration was assessed under normal and stressed conditions using microchemoattraction chambers. Effect of NO on the adhesion and migration was assessed with or without co-exposure of S-nitroso-N-acetylpenicillamine (SNAP). RESULTS: NO did not affect the degree of adhesion or migration of HTMC (p > 0.05). The degree of adhesion increased although the degree of migration decreased with 1% serum (p < 0.05). Degrees of migrations decreased after mechanical stress (p < 0.05). CONCLUSIONS: NO may not affect the adhesion or migration of HTMC.
