Polysorbate-80 modified neurotoxin nanoparticle with its transport and cytotoxicity against blood-brain barrier.
- Author:
Yan-min ZHAO
1
;
Ai-xiao XIA
;
Ying-hui WEI
;
Ye-ping RUAN
;
Fan-zhu LI
Author Information
1. College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Biological Transport;
Blood-Brain Barrier;
Brain;
blood supply;
Capillary Permeability;
Cell Survival;
drug effects;
Cells, Cultured;
Drug Carriers;
Electric Impedance;
Enbucrilate;
chemistry;
toxicity;
Endothelial Cells;
cytology;
metabolism;
Female;
Male;
Nanoparticles;
Neurotoxins;
administration & dosage;
pharmacokinetics;
Particle Size;
Polysorbates;
chemistry;
toxicity;
Rats;
Rats, Sprague-Dawley
- From:
Acta Pharmaceutica Sinica
2010;45(10):1312-1316
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at the transport across blood-brain barrier (BBB) of polysorbate-80 modified neurotoxin loaded polybutylcyanoacrylate nanoparticle (P-80-NT-NP) and its cytotoxicity. An in vitro model of BBB using rat brain microvascular endothelial cells (rBMECs) was established. The cytotoxicity of P-80-NT-NP was measured by the MTT assays, where neurotoxin (NT), nanoparticle (NP), neurotoxin nanoparticle (NT-NP) as control, and the permeability of P-80-NT-NP was determined by using of Millicell insert coculture with rBMECs and fluorescence spectrophotometry. MTT results showed that NT, NP, NT-NP and P-80-NT-NP were avirulent to rBMECs when the concentration of NT was lower than 200 ng x mL(-1). But the cytotoxicity of NP, NT-NP and P-80-NT-NP would be augmented accordingly as concentration increased (P < 0.01), causing obvious reductions of cell survival rate, with no significant difference between them (P > 0.05). When the concentration of NT was 150 ng x mL(-1), the permeability on rBMECs of P-80-NT-NP and NT-NP were both significantly higher than that of NT (P < 0.01), and the permeability of P-80-NT-NP was greater than that of NT-NP (P < 0.05). In conclusion, polysorbate-80 modified neurotoxin nanoparticles can transport across the BBB, while concentration of NT is greater than 200 ng x mL(-1), P-80-NT-NP has a little cytotoxicity against rBMECs.
