Preparation of ATP-2Na loaded liposome and its effect on tissues energy state in myocardial ischemic mice.
- Author:
Feng-mei PI
1
;
Xi-de TU
;
Yue WU
Author Information
1. China Pharmaceutical University, Nanjing 210009, China.
- Publication Type:Journal Article
- MeSH:
Adenosine Triphosphate;
administration & dosage;
blood;
metabolism;
Animals;
Cetrimonium Compounds;
chemistry;
Drug Carriers;
Liposomes;
chemistry;
Liver;
metabolism;
Male;
Mice;
Myocardial Ischemia;
blood;
metabolism;
Myocardium;
metabolism;
Particle Size;
Random Allocation;
Surface-Active Agents;
chemistry
- From:
Acta Pharmaceutica Sinica
2010;45(10):1322-1326
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study is to improve liposome encapsulation efficiency of water soluble drug ATP-2Na with hydrophobic ion pairing method, and evaluate its effect on tissues energy state in myocardial ischemia mice. Ion pair complex of ATP-2Na with HTAB was prepared first; then the liposomes were prepared by ethanol injection method. The size and zeta potential of ATP-2Na liposome were investigated. Its effect on tissues energy state in myocardium ischemia mice was evaluated by detecting ATP-2Na concentration in tissues and blood after injection in comparison to ATP-2Na solution. The diameters and zeta potential of ATP-2Na liposomes were (144.0 +/- 2.7) nm and (+16.2 +/- 1.6) mV, respectively. The encapsulation efficiency was (85.02 +/- 2.31) %. The in vitro drug release pattern from liposomes matches with Weibull equation. Compared with ATP-2Na solution, ATP-2Na liposome increased the ATP concentration of blood in myocardial ischemic mice very significantly; compared with blank, ATP-2Na liposome increased ATP content of myocardium and liver in myocaridal ischemic mice significantly, but ATP-2Na solution didn't show this effect. ATP-2Na liposome might have an advantage in improving tissue energy state on myocaridal ischemic animals.
