Effect of major components of maijunan tablets on the transport of hydrochlorothiazide in Caco-2 cell monolayer model.
- Author:
Xiao-Huan LIAO
1
;
Jun-Jun WANG
;
Ming-Yong GAO
;
Qian GAO
;
Yong CHEN
Author Information
1. Hubei Province Key Laboratory of Biotechnology of Chinese Traditional Medicine, Hubei University, Wuhan 430062, China.
- Publication Type:Journal Article
- MeSH:
ATP-Binding Cassette, Sub-Family B, Member 1;
antagonists & inhibitors;
Absorption;
drug effects;
Biological Transport, Active;
drug effects;
Caco-2 Cells;
Drugs, Chinese Herbal;
administration & dosage;
isolation & purification;
pharmacology;
Humans;
Hydrochlorothiazide;
pharmacokinetics;
Hydrogen-Ion Concentration;
Indole Alkaloids;
administration & dosage;
isolation & purification;
pharmacology;
Isoflavones;
administration & dosage;
isolation & purification;
pharmacology;
Plants, Medicinal;
chemistry;
Time Factors;
Vasodilator Agents;
administration & dosage;
isolation & purification;
pharmacology;
Verapamil;
pharmacology
- From:
Acta Pharmaceutica Sinica
2010;45(1):104-108
- CountryChina
- Language:Chinese
-
Abstract:
To study the effects of major components of Maijunan tablets, puerarin (Pue) and rhynchophylline (Rhy) on the transport of hydrochlorothiazide (Hct) Caco-2 cell monolayer model, the transport parameters of Hct, such as apparent permeability coefficient (P(app) (B --> A) and P(app) (A --> B)) and the ratio of P(app) (B --> A) versus P(app) (A --> B), were studied and compared when Hct was used solely and co-used with Pue and/or Rhy. The effects of drug concentrations, conveying times, P-glyprotein (P-gp) inhibitor verapamil and conveying Liq pH values on the transport of Hct in the above conditions were also investigated. The results indicated that the absorption of Hct in Caco-2 cell monolayer model could be a carrier-mediated active transport, along with the excretion action mediated by P-gp. Pue can decrease the excretion action of Hct mediated by P-gp, and Rhy had no significant effect on the transport of Hct. The co-use of Hct, Pue and Rhy enhanced the absorption of Hct. Meanwhile, conveying Liq pH value had significant influence on the transport of Hct. The absorption of Hct at pH 6.0 was higher than that at pH 7.4.
