Effects of dihydroxy-stilbene compound Vam3 on airway inflammation, expression of ICAM-1, activities of NF-kappaB and MMP-9 in asthmatic mice.
- Author:
Li YANG
1
;
Chun-suo YAO
;
Zhi-yuan WU
;
Ling-ling XUAN
;
Jin-ye BAI
;
Gui-fang CHENG
;
Mao LIN
;
Ming-chun WEN
;
Qi HOU
Author Information
1. Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Anti-Asthmatic Agents;
pharmacology;
Anti-Inflammatory Agents;
pharmacology;
Asthma;
chemically induced;
metabolism;
Benzofurans;
pharmacology;
Cell Line, Tumor;
Humans;
Inflammation;
metabolism;
Intercellular Adhesion Molecule-1;
metabolism;
Leukemia, Myeloid;
metabolism;
pathology;
Lung;
metabolism;
pathology;
Lung Neoplasms;
metabolism;
pathology;
Male;
Matrix Metalloproteinase 9;
metabolism;
Mice;
Mice, Inbred BALB C;
NF-kappa B;
metabolism;
Ovalbumin;
Stilbenes;
pharmacology
- From:
Acta Pharmaceutica Sinica
2010;45(12):1503-1508
- CountryChina
- Language:Chinese
-
Abstract:
The aim of the present study is to investigate the effects of Vam3 which is one of the dihydroxystilbene compounds on expressions of ICAM-1 in the lungs of OVA-induced asthmatic mice and the mechanisms of anti-airway inflammation. Balb/c mice were challenged with OVA inhalation. Lung tissues were stained with Mayer's hematoxylin and eosin for histopathologic examination. The expression of ICAM-1 in the lungs of mice was analyzed by Western blotting and immunohistochemistry method. The NF-kappaB activities were detected by NF-kappaB-luc reporter genetic transient transfection method. The activities of MMP-9 induced by LPS, TNF-alpha and PMA in THP-1 cells were determined by gelatin zymography method. The results showed that Vam3 could inhibit the expression of ICAM-1 in the OVA-induced mouse model. In addition, Vam3 could significantly suppress the activities of NF-kappaB in A549 cells and MMP-9 in THP-1 cells induced by LPS, TNF-alpha and PMA. These results suggested that Vam3 could alleviate the asthmatic inflammation by decreasing ICAM-1 expression in asthmatic mice, down regulating NF-kappaB and MMP-9 activities. Compound Vam3 showed inhibitory effects on inflammatory signal pathways involved in asthma.
