Simultaneous determination of 4 phenolic acids in cangerzi by ultra-performance liquid chromatography.
- Author:
Liu YANG
1
;
Zhi-jun SU
;
Shun-jun XU
;
Jin-xiong WU
;
Lu-lu CHEN
;
Ruo-long ZHOU
;
Xiong LI
;
Xing ZENG
Author Information
1. Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510120, China. yangliume@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Chlorogenic Acid;
analysis;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
analysis;
Fruit;
chemistry;
Hydroxybenzoates;
analysis;
Plants, Medicinal;
chemistry;
Quality Control;
Quinic Acid;
analogs & derivatives;
analysis;
Reproducibility of Results;
Xanthium;
chemistry
- From:
Acta Pharmaceutica Sinica
2010;45(12):1537-1540
- CountryChina
- Language:Chinese
-
Abstract:
In this study, an analytical method was developed and used to quantify simultaneously protocatechuic acid, neochlorogenic acid, cryptochlorogenic acid and 1, 3-dicaffeoylquinic acid--four bioactive compounds contained in Fructus Xanthii using UPLC. The contents of four phenolic components of 28 batches of samples collected from different product areas and markets were determined and compared by means of this established method. The mobile phase was composed of methanol and water containing 0.1% phosphoric acid. Chromatography was monitored at dual-wavelengths--220 and 327 nm. Flow rate was 0.4 mL x min(-1) and column temperature was 35 degrees C. The correlation coefficient between concentration and chromatographic peak area of protocatechuic acid, neochlorogenic acid, cryptochlorogenic acid and 1, 3-dicaffeoylquinic acid was over 0.9999 in the range of 0.3570-35.70, 2.500-250.0, 1.060-106.1, 1.010-101.0 microg x mL(-1), respectively. The average recoveries of the four compounds were 97.68%, 99.55%, 97.92% and 100.4%, respectively. In conclusion, the established method can rapidly attain an accurate and reproducible result used to control the quality of Fructus Xanthii.
