Simultaneous determination of peimisine and sipeimine in Fritillaria walujewii regel and Fritillaria pallidiflora Schrenk by UPLC-ELSD.
- Author:
Bao-zhong DUAN
1
;
Lin-fang HUANG
;
Shi-lin CHEN
Author Information
1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
- Publication Type:Journal Article
- MeSH:
Alkaloids;
analysis;
Cevanes;
analysis;
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
analysis;
Fritillaria;
chemistry;
Light;
Plant Roots;
chemistry;
Plants, Medicinal;
chemistry;
Quality Control;
Reproducibility of Results;
Scattering, Radiation
- From:
Acta Pharmaceutica Sinica
2010;45(12):1541-1544
- CountryChina
- Language:Chinese
-
Abstract:
The paper reports the establishment of a method for simultaneous determination of peimisine and sipeimine contents in Fritillaria walujewii Regel and Fritillaria pallidiflora Schrenk. The analyses were performed on an ultra-performance liquid chromatography with evaporative light scattering detection (UPLC-ELSD), equipped with a binary solvent manager, a sampler manager and a column compartment, and connected to Waters Empower 2 software. An Acquity UPLC BEH C18 column (100 mm x 2.1 mm, 1.7 microm) was used for all analysis. The investigated compounds were separated with gradient mobile phase consisting of acetonitrile-0.02% triethylamine-water. The temperature of sample manager was set at 25 degrees C. Drift tube temperature was 40 degrees C, and spray parameter was 40% with injection volume of 1 microL. The investigated compounds including peimisine and sipeimine had good linearity (r > or = 0.9991) over the tested ranges. The average recovery was 94.5% and 98.1% with RSD < or = 2.36%. The UPLC-ELSD method is simple, sensitive and accurate with good repeatability, which is available for quality control of F. walujewii Regel and F. pallidiflora Schrenk.
