Screening and application of human-derived HBsAg bacteriophage single chain antibody in clinical diagnosis.
- Author:
Yanwei ZHONG
1
;
Jun CHENG
;
Shuangshuang SHI
;
Jingmin ZHAO
;
Gang WANG
;
Xiaobing XIA
;
Xiaojun TIAN
;
Li LI
;
Lingxia ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Bacteriophages; metabolism; Enzyme-Linked Immunosorbent Assay; Hepatitis B Antibodies; Hepatitis B Surface Antigens; immunology; Hepatitis B, Chronic; diagnosis; Humans; Peptide Library
- From: Chinese Journal of Experimental and Clinical Virology 2002;16(3):223-225
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify human single chain Fv antibody (ScFv) against hepatitis B viral surface antigen.
METHODSThe recombinant phages were panned by HBsAg which was coated in a microtiter plate, after five rounds of biopanning, 56 phage clones were identified specific to HBsAg. The specificity of ScFv was evaluated by ELISA and immunohistochemistry, respectively.
RESULTSThe data of HB sAg-ScFv DNA digestion and DNA sequencing showed that the ScFv gene is composed of 750 bp. ELISA and immunohistochemistry demonstrated that the human single chain Fv antibody against hepatitis B surface antigen has a specific combination character with hepatitis B surface antigen of different sources and paraffin-embedded patients tissue specimens, it did not react with normal liver tissue and HCV.
CONCLUSIONSThe application of HBsAg specific ScFv in immunohistochemistry was successfully achieved.
