Nucleotide sequence analysis of new genotype of hepatitis G virus in population at high risk for HCV infection in Guangxi.
- Author:
Xianmin GE
1
;
Danya LI
;
Rongrong WU
;
Guoyong HUANG
;
Haidong PAN
;
Kun CAO
;
Pingchuan LI
;
Shusheng WANG
;
Mizokami MASASHI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Blood Donors; China; epidemiology; Female; GB virus C; genetics; isolation & purification; Genotype; Hepatitis C; epidemiology; Humans; Liver Diseases; virology; Male; Polymerase Chain Reaction; RNA, Viral; genetics; Sequence Analysis, RNA; Substance Abuse, Intravenous; virology
- From: Chinese Journal of Experimental and Clinical Virology 2002;16(3):242-245
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo examine the prevalence and the sequence of the genes of new genotypes of hepatitis G virus (HGV) in Guangxi, China.
METHODSSerum samples were collected from 85 intravenous drug abusers (IVDAs), 80 patients with liver diseases (PLDs) and 50 blood donors (BDs). All sera (n=215) were tested by using EIA for HBsAg, anti-HCV and anti-HIV, and by using nested PCR for HGV RNA. In 62 subjects positive for HGV, HGV RNA was sequenced, and a phylogenetic tree was constructed for analyzing genotypes of HGV.
RESULTSHGV RNA was detected in 85 of 215 serum samples (39.53%). The positivity rates for HBsAg, anti-HCV and anti-HIV were 39.07%, 42.79% and 0, respectively. First, 11 nucleotide sequences were determined and the isolates were grouped into three clusters with HGV. 5 of 11 HGV isolates clustered in a distinct phylogenetic branch (genotype Asia) which was different from the described GBV-C and HGV sequences, suggesting the presence of a new genotype of HGV in this locality. Second, 51 nucleotide sequences were determined and analyzed for their genotypes of HGV, and showed genotype GBV-C (3.23%), genotype HGV 30-65% and new genotype (genotype Asia) 64.51%, respectively.
CONCLUSIONSThere were subgenotypes in 3 genotypes of HGV; The predominant genotypes of HGV were genotype Asia and genotype HGV among IVDAs, PLDs, and BDs patients in Guangxi, China.
