Inhibition of Luciferase expression in mammalian cells by AAV vector plasmid mediated Luciferase shRNA.
- Author:
Xin MA
1
;
Xiaochun LU
;
Jainqiang PENG
;
Shuping TAN
;
Zhenhua YUAN
;
Fang YIN
;
Hong WANG
;
Xiaobing WU
;
Yunde HOU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Gene Expression; Genetic Vectors; Humans; Luciferases; biosynthesis; genetics; Plasmids; genetics; Promoter Regions, Genetic; RNA; genetics; Transfection
- From: Chinese Journal of Experimental and Clinical Virology 2002;16(3):253-255
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEConstructing a plasmid containing the shRNA of luciferase to suppress the expression of luciferase in BHK-21 cell.
METHODSA 334 bp human U6 snRNA promoter was amplified from human genomic DNA by PCR and ligated to a 21 bp reverse repeated motif of luciferase target sequence with 9 bp spacer and AAV plasmid pSNAV. The recombinant pSNAV/U6/Luc plasmid cotransfected with pMAMneoLuc or transfected luciferase cell line to detect the effect of luciferase expression separately.
RESULTSpSNAV/U6/luc suppresses the luciferase expression from pMAMneoLuc by 50% and luciferase cell line by 70%.
CONCLUSIONSThe results showed that the short hairpin RNA of luciferase can efficiently suppress its expression in BHK-21.
