Preparation and application of monoclonal antibodies to recombinant human IFN alpha.
- Author:
Meiying WU
1
;
Yanping AI
;
Ren REN
;
Yixin LIANG
;
Jie LI
;
Wenguang SONG
;
Dajun YANG
;
Jianbo SUN
;
Yongqing CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Monoclonal; biosynthesis; Antibody Affinity; Antibody Specificity; Cross Reactions; Enzyme-Linked Immunosorbent Assay; Hybridomas; secretion; Interferon Type I; immunology; isolation & purification; Male; Mice; Mice, Inbred BALB C; Recombinant Proteins
- From: Chinese Journal of Experimental and Clinical Virology 2002;16(3):261-263
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo prepare and apply monoclonal antibodies (McAb) against recombinant human interferon alpha (rHu IFN-alpha).
METHODSFive cell lines (2E9, 4G1, 2A7, 2C9, 4G10) secreting McAbs against rHu IFN-alpha were established by hybridoma technique.
RESULTSAll the cell lines secreted monoclonal antibodies stably. Functions of secreting antibodies of the five cell lines lasted for 6 months in BALB/c mice and 8 months in cell culture. The specificity of antibody was constant. The Ig subclasses of the McAbs were IgG1. Anti-IFN McAb affinity purification column was prepared by coupling the anti IFN-alpha McAb to Sepharose 4B. The combining rate reached was higher than 95%.
CONCLUSIONSThe highest purification efficiency was obtained by using 4G10 column.
