Establishment of a double-antigen sandwich ELISA for detecting total antibodies to human immunodeficiency virus type 1/2.
- Author:
Hongxia HE
1
;
Panyong MAO
;
Jun HOU
;
Shiwen HONG
;
Lei ZHU
;
Yan HU
;
Yanping BAI
Author Information
- Publication Type:Journal Article
- MeSH: Enzyme-Linked Immunosorbent Assay; methods; HIV Antibodies; blood; HIV Infections; blood; virology; HIV-1; immunology; HIV-2; immunology; Humans
- From: Chinese Journal of Experimental and Clinical Virology 2002;16(3):288-291
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo describe and evaluate a double-antigen sandwich ELISA for detecting human immunodeficiency virus type 1/2 (HIV-1/2) specific antibodies.
METHODSThe peptides gp41.1(sp1), gp41.2(sp2), gp120(sp3) and p24(sp4) of HIV-1 and gp36(sp5) of HIV-2 were artificially synthesized. Then sp1, sp3, sp4 and sp5 were used as coating antigens; sp1, sp2, sp4 and sp5 labeled with HRP were used as conjugates in this sandwich ELISA.
RESULTSThe specificity and sensitivity of the assay were both 100% in detecting anti-HIV of 40 control sera of the second generation panel, higher than indirect ELISA (specificity 90% and sensitivity, 65%, respectively). All of 210 sera from individuals with other diseases were negative for anti-HIV. The consistency rate was 100% when our sandwich ELISA and Abbott HIVAB were used to detect anti-HIV in 90 healthy blood donors and 88 HIV infected individuals.
CONCLUSIONSThe results showed that this sandwich ELISA for detection of anti-HIV is specific, sensitive and convenient, and it is suitable for screening blood donors and detecting HIV infection.
