Molecular characterization of hantavirus Shandong isolate JNL virus strain.
- Author:
Zhi-hui YAO
1
;
Yong-xin YU
;
Guan-mu DONG
;
Xiang-zhi MENG
;
Zhao-hang JIN
Author Information
- Publication Type:Journal Article
- MeSH: DNA, Viral; analysis; Hantaan virus; classification; genetics; isolation & purification; Hemorrhagic Fever with Renal Syndrome; virology; Humans; Phylogeny; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid
- From: Chinese Journal of Experimental and Clinical Virology 2003;17(2):112-115
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo understand the molecular epidemiologic characteristics of hantavirus Shandong isolate JNL virus strain.
METHODSThe complete M and S gene of the JNL virus isolated from Shandong Province was amplified by RT- PCR, and the purified PCR product was cloned into T vector for sequencing.
RESULTSThe results revealed that the JNL M segment was 3615 bp in length, encoding 1135 amino acids, and the S segment was 1698 bp encoding 429 amino acids, JNL belongs to HTN virus. The comparison of homology with HTN and SEO types showed that the difference of M and S complete sequences between JNL and all other HTN virus strains reached 20.0%-20.6%, and 15.5%-16.0%, respectively. Phylogenetic tree also showed that the position of JNL is located at a different clade.
CONCLUSIONSHTN virus Shandong local isolate JNL strain is a new specific HTN subtype virus.
