Polymorphism of CXCR4 coding region of human immunodeficiency virus-1 in Chinese Han people.
- Author:
Ming-xu LIU
1
;
Fu-sheng WANG
;
Wei-guo HONG
;
Bo WANG
;
Lei JIN
;
Zhou-yun LEI
;
Jing HOU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Asian Continental Ancestry Group; Base Sequence; China; ethnology; Female; Gene Frequency; HIV-1; genetics; Humans; Male; Middle Aged; Molecular Sequence Data; Point Mutation; Polymorphism, Single Nucleotide; Receptors, CXCR4; genetics
- From: Chinese Journal of Experimental and Clinical Virology 2003;17(2):162-164
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the polymorphism of human immunodeficiency virus (HIV)-1 coreceptor CXCR4 in Chinese Han ethnic group for AIDS prevention and treatment.
METHODSTotally 48 individuals were enrolled into the study. CXCR4 (cDNA No-AF147204) was cloned by PCR amplification using 2 pairs of primers, then sequenced using sequencing primers. The results of the same sequencing primers were analyzed by DNAstar software to find and identify single nucleotide polymorphism (SNP) sites.
RESULTSTotally 7 SNPs were found in the coding region of CXCR4, among them 3 were synonymous mutation (C-->T at loci 129, 426 and 968), 3 were missense mutation (C-->T at locus 38, A-->T at locus 90, and A-->C at locus 712) and 1 was stop mutation (C-->T at 106, which converted the codon for glutamic acid into stop codon).
CONCLUSIONSThe polymorphism of CXCR4 coding region in Chinese Han is probably different from that of the other ethnic groups. Six of the 7 SNPs were discovered for the first time. Their influences on AIDS progression are worthy of studying.
