Sequence analysis of VP7 gene from rotavirus field strain from Guangzhou, China.
- Author:
Dong-mei CHEN
1
;
Yuan QIAN
;
You ZHANG
;
Ru-xu CHANG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Antigens, Viral; Capsid Proteins; genetics; Diarrhea, Infantile; virology; Humans; Infant; Molecular Sequence Data; Phylogeny; Reverse Transcriptase Polymerase Chain Reaction; Rotavirus; classification; isolation & purification; Rotavirus Infections; virology; Sequence Analysis; Serotyping
- From: Chinese Journal of Experimental and Clinical Virology 2003;17(2):165-168
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the difference of VP7 fragment at the nucleotide level between rotavirus Guangzhou field strain R97-196 and rotavirus Beijing field strain or prototype strains.
METHODSThe VP7 fragment amplified from Guangzhou field strain R97-196 by RT-PCR was cloned into the T-A cloning vector pUCm-T and sequenced.
RESULTSThe VP7 fragment from Guangzhou field strain R97-196 was 1,062 bp in length and contained two open reading frames which is consistent with that reported in the literature. The sequence analysis revealed that the cDNA from R97-196 shared higher nucleotide and amino acid identities with Beijing field strain T73 (98% and %, respectively) than with serotype 2-4 (G types) rotavirus (from 74% to 77% and 73% to 81%, respectively). The divergence of amino acid sequences is mainly within the nine divergence regions. The phylogenetic analysis revealed that the VP7 fragment from R97-196 was far away from rotavirus serotype G1 strain Wa.
CONCLUSIONSThe VP7 gene fragment from rotavirus Guangzhou field strain R97-196 belongs to rotavirus serotype G1. And variation of rotavirus VP7 gene fragment seems to be a geographic matter.