Quantitative HPLC analysis of main effective constituents and integrated chemical quality index(ICQI)in Dida.
- Author:
Xiao-Quan YANG
1
;
Yong-Qiang GUO
1
;
Cong-Long XIA
1
Author Information
- Publication Type:Journal Article
- Keywords: Dida; HPLC-DAD; integrated chemical quality index(ICQI); integrated evaluation; multiple wavelength detection
- From: China Journal of Chinese Materia Medica 2016;41(9):1685-1692
- CountryChina
- Language:Chinese
- Abstract: "Dida" is one of the most representative commonly used Tibetan medicines in treatment of hepatobilaiary diseases. To analyze and compare the content of 7 effective constituents in 11 species which are used as Dida. Integrated evaluation of them, which supply a basis for the resources exploitation for them. In this paper, most appropriate absorption wavelengths of different natural iridoid compounds, xanthones and oleanolic acid have been performed by using 3D spectrum technique. According to the results, the wavelength 210, 240 and 265 nm were used for detection of 7 effective constituents. By means of calculation, determination of 7 effective constituents indicated good linearity over the linear range with coefficients (r) of 0.999 9 respectively. The recoveries were found in the average range of 99% to 101% with RSD being less than 3%. Normalize and integrate by three-dimension the data. Get their integrated chemical quality index(ICQI). Results suggested that these plants used as Dida in Tibet medicine mostly contain these 7 effective constituents, but the different is notable. The integrated evaluation by the integrated chemical quality index (ICQI) has reference value for quality control.
