Astragali Radix and Hedysari Radix molecular identification of SSR primers screening and fingerprints code.

Author: Yi-Qing HOU ¹ ; You-Tai SHI ¹ ; Yan-Ping ZHANG ¹ ; Xin-Xing LIU ¹ ; Yu-Liang CHEN ¹
Author Information

1. Gansu Province Academy of Agricultural Sciences, Biotechnology Research Institute, Lanzhou 730070, China.
Publication Type:Journal Article
Keywords: Astragali Radix; Hedysari Radix; SSR; clustering analysis; molecular identification
From: China Journal of Chinese Materia Medica 2016;41(10):1819-1822
CountryChina
Language:Chinese
Abstract: Leguminous related SSR primers were collected, core primers used for Astragali Radix and Hedysari Radix identification were screened and validated by using molecular marker techniques. 6 core primers were selected from 101 pairs of primers, the molecular weight of PCR products was 100-500 bp, which formed 7-12 electrophoresis bands with 55 amplified loci. The percentage of polymorphic loci was 100%, and the average polymorphism information content was 0.371. According to the results of cluster analysis, obtained core primer could completely distinguish 62 mixture samples of Astragali Radix and Hedysari Radix in similarity coefficient of 0.46. Core primers and the corresponding characteristics from gel electrophoresis were tagged. The results provide identification basis for Astragali Radix and Hedysari Radix.