Triton X-100 induces apoptosis in human hepatoma cell lines.
- Author:
Ju Mi AHN
1
;
Se Jong KIM
;
Hoguen KIM
;
Chanil PARK
;
Won Ho KIM
;
Jeon Han PARK
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: Triton X-100; apoptosis; hepatoma cells lines; DNA fragmentation; TUNEL test; LDH Introduction
- MeSH: Apoptosis*; Carcinoma, Hepatocellular/pathology*; Carcinoma, Hepatocellular/genetics; DNA Fragmentation; Detergents/pharmacology*; Human; Liver Neoplasms/pathology*; Liver Neoplasms/genetics; Octoxynol/pharmacology*; Tumor Cells, Cultured/drug effects
- From:Yonsei Medical Journal 1997;38(1):52-59
- CountryRepublic of Korea
- Language:English
- Abstract: The detergent Triton X-100 was used to establish a model for apoptosis in hepatoma cell lines. The electrophoresis of DNA extracted from 0.01% Triton X-100 treated hepatoma cell lines showed DNA ladder formation, a hallmark of apoptosis. The DNA fragmentation appeared within less than 60 min of the Triton X-100 treatment. Chromatin condensation and apoptotic bodies were observed by hematoxylin and eosin (H & E) stain, and fragmented nucleosome was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling (TUNEL) test. Apoptosis was semi-quantitated by measuring the lactate dehydrogenase (LDH) level for cytotoxity. It was found that apoptosis had been induced in more than 90% of the cells treated with Triton X-100 for 150 min. These data show that Triton X-100 efficiently induces the apoptotic cell death in hepatoma cell lines.
