Chemical constituents from endophyte Chaetomium globosum in Imperata cylindrical.
- Author:
Li SHEN
;
Li ZHU
;
Zhong-qi WEI
;
Xiao-wen LI
;
Ming LI
;
Yong-chun SONG
- Publication Type:Journal Article
- MeSH:
Biological Factors;
chemistry;
pharmacology;
Cell Line;
Cell Survival;
drug effects;
Chaetomium;
chemistry;
Endophytes;
chemistry;
Humans;
Molecular Structure;
Poaceae;
microbiology;
Spectrometry, Mass, Electrospray Ionization
- From:
China Journal of Chinese Materia Medica
2015;40(23):4645-4649
- CountryChina
- Language:Chinese
-
Abstract:
Isolation and purification of chemical constituents from solid culture of endophyte Chaetomium globosum in Imperata cylindrical was performed through silica gel column chromatography, gel filtration over Sephadex LH-20 and preparative HPLC. Nine compounds were obtained and their structures were determined as chaetoglobosin F(1), chaetoglobosin Fex(2), chaetoglobosin E(3) cytoglobosin A(4), penochalasin C(S), isochaetoglobosin D (6), N-benzoylphenylalaninyl-N-benzoyphenylalaninate(7), uracil(8) and 5-methyluracil(9), respectively, based on HR-MS and NMR data and comparison with literatures. Compound 7 was isolated from Chaeeomium sp. for the first time. In vitro cytotoxicity of compounds was evaluated using MTT mothed and 1,3,4 and 5 showed inhibition activity to the human cervical carcinoma cell HeLa with IC50 values of 99.43, 23.77, 97.92, 86.25 micromol x L(-1), while positive cotolocisnin Ad apno1ch alse IC50 24.33 micromol x L(-1).
