Screening of meaningful endophytic fungi in Dendrobium officinale based on polysaccharides and flavonoids.
- Author:
Meng-Meng YU
1
;
Ling-Shang WU
1
;
Jin-Ping SI
1
;
Jing-Jing LIU
1
;
Zhi-Xiang YU
1
;
Hua-Cheng WANG
1
;
Min ZHANG
2
;
Song RONG
3
Author Information
- Publication Type:Journal Article
- Keywords: Dendrobium officinale; HPLC; endophytic fungi; flavonoids ingredients; monosaccharide composition
- From: China Journal of Chinese Materia Medica 2016;41(12):2208-2212
- CountryChina
- Language:Chinese
- Abstract: To reveal the relationship between endophytic fungi and the functional components, saccharides and flavonoids in the mycelia or fermented liquor of 21 endophytic fungi in D.officinale were detected using HPLC and UV spectrophotometer.The results showed that the ethyl acetate extracts from 21 fungal strains all contain flavonoids.According to the chromatographic retention time of HPLC and UV spectra characteristics of flavonoids, strain DO49 was found produce naringenin, strains DO23, DO81 and DO83 were found produce rutin.The water-soluble extracts from 21 strains all had polysaccharides.However, there was difference in the composition of monosaccharides derived from polysaccharides among different strains.According to the composition of monosaccharides and the peak area ratio of mannose and glucose, the fungal strains including DO23, DO26, DO81, DO54, DO55, DO83 product polysaccharides associated with D.officinale were selected.In conclusion, based on the saccharides and flavonoids, the excellent endophytic fungal strains DO23, DO81 and DO83 were selected, which could produce the same flavonoids and similar polysaccharides in D.officinale.
