Expressions of E-cadherin, p120ctn, β-catenin and NF-κB in ulcerative colitis.
10.1007/s11596-015-1439-9
- Author:
Chao ZHANG
1
;
Li-Wei LIU
;
Wen-Jia SUN
;
Sheng-Hui QIN
;
Ling-Zhi QIN
;
Xi WANG
Author Information
1. Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China, 502826954@qq.com.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Cadherins;
metabolism;
Catenins;
metabolism;
Colitis, Ulcerative;
metabolism;
pathology;
Down-Regulation;
Female;
Humans;
Male;
Middle Aged;
NF-kappa B;
metabolism;
Statistics, Nonparametric;
Young Adult;
beta Catenin;
metabolism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2015;35(3):368-373
- CountryChina
- Language:English
-
Abstract:
This study was aimed to investigate the expressions of E-cadherin, p120ctn, β-catenin and NF-κB in ulcerative colitis (UC) tissues and the implications of their expressions in the pathogenesis of UC. The expressions of E-cadherin, p120ctn, β-catenin and NF-κB were detected by immunohistochemistry, and those of p120ctn and NF-κB by Western blotting in 23 cases of UC and 17 cases of normal colonic tissues. The relationship between the expression of E-cadherin or NF-κB and that of p120ctn was analyzed by Spearman rank correlation analysis. The results showed that in UC and normal colonic groups, the abnormal expression rate of E-cadherin, p120ctn, β-catenin, and NF-κB was 52.2% vs. 0 (P<0.05), 73.9% vs. 23.5% (P<0.05), 65.2% vs. 17.6% (P<0.05) and 78.4% vs. 23.5% (P<0.05), respectively. p120ctn expression was positively correlated with E-cadherin expression (r=0.404, P<0.05), but negatively with nuclear NF-κB expression (r= - 0.347, P<0.05). Western blotting showed that as compared with the normal controls, the p120ctn protein level was significantly decreased (P<0.05), whereas the NF-κB protein level was increased (P<0.05) in UC tissues. It was concluded that in the colonic tissues of UC patients, the expressions of E-cadherin, p120ctn and β-catenin are decreased, suggesting the mucosal barrier is impaired in UC. Moreover, NF-κB is increased and activated in the UC tissues, resulting in the inflammation in UC. p120ctn may influence the UC development through modulating intercellular adhesion and inflammatory response.
