Resveratrol-downregulated phosphorylated liver kinase B1 is involved in senescence of acute myeloid leukemia stem cells.
10.1007/s11596-015-1457-7
- Author:
Dan-Yue PENG
1
;
Hui SONG
;
Ling-Bo LIU
Author Information
1. Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China, 962040850@qq.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Cellular Senescence;
Cyclin-Dependent Kinase Inhibitor p21;
genetics;
Down-Regulation;
Gene Expression Regulation, Neoplastic;
drug effects;
Humans;
Leukemia, Myeloid, Acute;
enzymology;
genetics;
pathology;
Neoplastic Stem Cells;
drug effects;
Phosphorylation;
Protein-Serine-Threonine Kinases;
genetics;
metabolism;
Sirtuin 1;
genetics;
metabolism;
Stilbenes;
pharmacology
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2015;35(4):485-489
- CountryChina
- Language:English
-
Abstract:
Senescence is an important obstacle to cancer development. Engaging a senescent response may be an effective way to cure acute myeloid leukemia (AML). The aim of this study was to examine the effect of resveratrol-downregulated phosphorylated liver kinase B1 (pLKB1) on the senescence of acute myeloid leukemia (AML) stem cells. The protein expressions of pLKB1 and Sirtuin 1 (SIRT1), a regulator of pLKB1, were measured in CD34(+)CD38(-) KG1a cells treated with resveratrol (40 μmol/L) or not by Western blotting. Senescence-related factors were examined, including p21 mRNA tested by real-time PCR, cell morphology by senescence-associated β-galactosidase (SA-β-gal) staining, cell proliferation by MTT assay and cell cycle by flow cytometry. Besides, apoptosis was flow cytometrically determined. The results showed that pLKB1 was highly expressed in CD34(+)CD38(-) KG1a cells, and resveratrol, which could downregulate pLKB1 through activation of SIRT1, induced senescence and apoptosis of CD34(+)CD38(-) KG1a cells. It was concluded that resveratrol-downregulated pLKB1 is involved in the senescence of AML stem cells.
