Effects of β-catenin on differentially expressed genes in multiple myeloma.
10.1007/s11596-015-1468-4
- Author:
Hui CHEN
1
;
Wei CHAI
;
Bin LI
;
Ming NI
;
Guo-Qiang ZHANG
;
Hua-Wei LIU
;
Zhuo ZHANG
;
Ji-Ying CHEN
;
Yong-Gang ZHOU
;
Yan WANG
Author Information
1. Department of Orthopedics, Chinese People's Liberation Army General Hospital, Beijing, 100853, China, chenhui301@126.com.
- Publication Type:Journal Article
- MeSH:
CDC2 Protein Kinase;
Cyclin B1;
genetics;
Cyclin-Dependent Kinases;
genetics;
Forkhead Box Protein M1;
Forkhead Transcription Factors;
genetics;
Gene Expression Profiling;
methods;
Gene Expression Regulation, Neoplastic;
Gene Regulatory Networks;
Gene Silencing;
Humans;
Multiple Myeloma;
genetics;
Oncogene Proteins v-fos;
genetics;
Protein Interaction Maps;
Proto-Oncogene Proteins c-jun;
genetics;
beta Catenin;
genetics
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2015;35(4):546-552
- CountryChina
- Language:English
-
Abstract:
This study aimed to identify the differentially expressed genes after silencing of β-catenin in multiple myeloma transduced with β-catenin shRNA. The DNA microarray dataset GSE17385 was downloaded from Gene Expression Omnibus, including 3 samples of MM1.S (human multiple myeloma cell lines) cells transduced with control shRNA and 3 samples of MM1.S cells transduced with β-catenin shRNA. Then the differentially expressed genes (DEGs) were screened by using Limma. Their underlying functions were analyzed by employing Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. Moreover, DEGs annotation was conducted based on the databases of tumor associated genes, tumor suppressed genes and the transcriptional regulation from patterns to profiles. Furthermore, the protein-protein interaction (PPI) relationship was obtained from STRING and the protein-protein interaction network and the functional modules were visualized by Cytoscape. Then, the pathway enrichment for the DEGs in the functional module was performed. A total of 301 DEGs, including 124 up-regulated and 117 down-regulated DEGs, were screened. Functional enrichment showed that CCNB1 and CDK1 were significantly related to the function of cell proliferation. FOS and JUN were related to innate immune response-activating signal transduction. Pathway enrichment analysis indicated that CCNB1 and CDK1 were most significantly enriched in the pathway of cell cycle. Besides, FOS and JUN were significantly enriched in the Toll-like receptor signaling pathway. FOXM1 was identified as a transcription factor. Moreover, there existed interactions among CCNB1, FOXM1 and CDK1 in PPI network. The expression of FOS, JUN, CCNB1, FOXM1 and CDK1 may be affected by β-catenin in multiple myeloma.
