A novel schiff base zinc coordination compound inhibits proliferation and induces apoptosis of human osteosarcoma cells.
10.1007/s11596-015-1493-3
- Author:
Ming YAN
1
;
Li PANG
2
;
Tan-tan MA
3
;
Cheng-liang ZHAO
4
;
Nan ZHANG
5
;
Bing-xin YU
6
;
Yan XIA
7
Author Information
1. Department of Spine Surgery, The First Hospital of Jilin University, Changchun, 130021, China. 514329822@qq.com.
2. Department of Emergency Medicine, The First Hospital of Jilin University, Changchun, 130021, China. Pangli211@163.com.
3. Department of Gastroenterology, The First Hospital of Jilin University, Changchun, 130021, China.
4. Affiliated Hospital of Chengde Medical College, Chengde, 067000, China.
5. Department of Emergency Medicine, The First Hospital of Jilin University, Changchun, 130021, China. zn0972@163.com.
6. Department of Ultrasonography, The China-Japan Union Hospital of Jilin University, Changchun, 130021, China.
7. Department of Gastroenterology, The First Hospital of Jilin University, Changchun, 130021, China. 466634002@qq.com.
- Publication Type:Journal Article
- Keywords:
anti-tumor activity;
apoptosis;
bone sarcoma MG-63 cells;
mitochondrial membrane potential;
schiff base zinc coordination compound
- MeSH:
Antineoplastic Agents;
chemical synthesis;
pharmacology;
Apoptosis;
drug effects;
Caspase 3;
genetics;
metabolism;
Caspase 8;
genetics;
metabolism;
Caspase 9;
genetics;
metabolism;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Cell Survival;
drug effects;
Coordination Complexes;
chemical synthesis;
pharmacology;
Fas Ligand Protein;
genetics;
metabolism;
G1 Phase Cell Cycle Checkpoints;
drug effects;
Gene Expression Regulation, Neoplastic;
drug effects;
Humans;
Membrane Potential, Mitochondrial;
drug effects;
Mitochondria;
drug effects;
metabolism;
pathology;
Osteoblasts;
drug effects;
metabolism;
pathology;
Proto-Oncogene Proteins c-bcl-2;
genetics;
metabolism;
Schiff Bases;
chemistry;
Signal Transduction;
Zinc;
chemistry;
bcl-2-Associated X Protein;
genetics;
metabolism;
fas Receptor;
genetics;
metabolism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2015;35(5):700-706
- CountryChina
- Language:English
-
Abstract:
Various kinds of schiff base metal complexes have been proven to induce apoptosis of tumor cells. However, it remains largely unknown whether schiff base zinc complexes induce apoptosis in human cancer cells. Here, we synthesized a novel schiff base zinc coordination compound (SBZCC) and investigated its effects on the growth, proliferation and apoptosis of human osteosarcoma MG-63 cells. A novel SBZCC was synthesized by chemical processes and used to treat MG-63 cells. The cell viability was determined by CCK-8 assay. The cell cycle progression, mitochondrial membrane potential and apoptotic cells were analyzed by flow cytometry. The apoptosis-related proteins levels were determined by immunoblotting. Treatment of MG-63 cells with SBZCC resulted in inhibition of cell proliferation and cell cycle arrest at G1 phase. Moreover, SBZCC significantly reduced the mitochondrial membrane potential and induced apoptosis, accompanied with increased Bax/Bcl-2 and FlasL/Fas expression as well as caspase-3/8/9 cleavage. Our results demonstrated that the synthesized novel SBZCC could inhibit the proliferation and induce apoptosis of MG-63 cells via activating both the mitochondrial and cell death receptor apoptosis pathways, suggesting that SBZCC is a promising agent for the development as anticancer drugs.
