Repetitive magnetic stimulation promotes neural stem cells proliferation by upregulating MiR-106b in vitro.
10.1007/s11596-015-1505-3
- Author:
Hua LIU
1
;
Xiao-hua HAN
2
;
Hong CHEN
3
;
Cai-xia ZHENG
3
;
Yi YANG
4
;
Xiao-lin HUANG
5
Author Information
1. Department of Rehabilitation Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. hualiuhua2005@163.com.
2. Department of Rehabilitation Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. 491695008@qq.com.
3. Department of Rehabilitation Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
4. College of Health Science, Wuhan Institute of Physical Education, Wuhan, 430079, China.
5. Department of Rehabilitation Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. xiaolinh2-006@126.com.
- Publication Type:Journal Article
- Keywords:
EdU;
cyclin-dependent kinase;
cyclin-dependent kinase inhibitor;
ki67;
microRNA-106b;
neural stem cells;
repetitive magnetic stimulation
- MeSH:
Animals;
Animals, Newborn;
Biomarkers;
metabolism;
Cell Proliferation;
genetics;
Cyclin-Dependent Kinase 2;
genetics;
metabolism;
Cyclin-Dependent Kinase 4;
genetics;
metabolism;
Cyclin-Dependent Kinase Inhibitor p21;
genetics;
metabolism;
Cyclin-Dependent Kinase Inhibitor p57;
genetics;
metabolism;
Cyclins;
genetics;
metabolism;
Gene Expression Regulation;
Hippocampus;
cytology;
metabolism;
Ki-67 Antigen;
genetics;
metabolism;
Magnetic Fields;
MicroRNAs;
genetics;
metabolism;
Neural Stem Cells;
cytology;
metabolism;
Primary Cell Culture;
Rats;
Rats, Sprague-Dawley;
Signal Transduction
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2015;35(5):766-772
- CountryChina
- Language:English
-
Abstract:
Neural stem cells (NSCs) proliferation can be influenced by repetitive transcranial magnetic stimulation (rTMS) in vivo via microRNA-106b-25 cluster, but the underlying mechanisms are poorly understood. This study investigated the involvement of microRNA-106b-25 cluster in the proliferation of NSCs after repetitive magnetic stimulation (rMS) in vitro. NSCs were stimulated by rMS (200/400/600/800/1000 pulses per day, with 10 Hz frequency and 50% maximum machine output) over a 3-day period. NSCs proliferation was detected by using ki-67 and EdU staining. Ki-67, p21, p57, cyclinD1, cyclinE, cyclinA, cdk2, cdk4 proteins and miR-106b, miR-93, miR-25 mRNAs were detected by Western blotting and qRT-PCR, respectively. The results showed that rMS could promote NSCs proliferation in a dose-dependent manner. The proportions of ki-67+ and Edu+ cells in 1000 pulses group were 20.65% and 4.00%, respectively, significantly higher than those in control group (9.25%, 2.05%). The expression levels of miR-106b and miR-93 were significantly upregulated in 600-1000 pulses groups compared with control group (P<0.05 or 0.01 for all). The expression levels of p21 protein were decreased significantly in 800/1000 pulses groups, and those of cyclinD1, cyclinA, cyclinE, cdk2 and cdk4 were obviously increased after rMS as compared with control group (P<0.05 or 0.01 for all). In conclusion, our findings suggested that rMS enhances the NSCs proliferation in vitro in a dose-dependent manner and miR-106b/p21/cdks/cyclins pathway was involved in the process.
