DNA methylation and histone modification relate to RASSF1A gene deletion in laryngeal carcinoma tissues.
- Author:
Jing YANG
1
;
Wen-yue JI
;
Ya-rong QU
;
Li-xia HE
;
Xu-dong ZHAO
;
Ming-zhu JIN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; CpG Islands; DNA Methylation; Female; Gene Deletion; Gene Expression Regulation, Neoplastic; Gene Silencing; Histones; genetics; metabolism; Humans; Laryngeal Neoplasms; genetics; metabolism; Male; Middle Aged; Neoplasm Staging; Promoter Regions, Genetic; Tumor Suppressor Proteins; genetics
- From: Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2011;46(4):308-312
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between RASSF1A gene expression and DNA methylation or histone modification in laryngeal carcinoma tissues.
METHODSChromatin immunoprecipitation (ChIP), methylation specific polymerase chain reaction (MSP) and realtime quantitative reverse transcription polymerase chain reaction (realtime RT-PCR) were used to analyze RASSF1A gene promoter region histone H3 lysine 9 methylation, H3 lysine 4 methylation, H3 lysine 9 acetylation, DNA methylation, and RASSF1A gene expression in laryngeal carcinoma tissue of 50 cases.
RESULTSDNA methylation rate of gene RASSF1A was 62% in 50 cases of laryngeal carcinoma, but no DNA methylation was found in normal control group, with a significant difference (χ(2) = 15.381, P < 0.05). DNA methylation had no correlation with age, gender, differentiation degree, T stage, pathological type and lymph node metastasis (P > 0.05). The affection of DNA methylation group was more than unmethylation group to expression of gene RASSF1A (t = -3.108, P < 0.01). There was positive correlation between RASSF1A deletion and gene hypermethylation or between H3 lysine 9 methylation of RASSF1A gene promoter and DNA methylation in laryngeal carcinoma tissue(r = 0.816, P < 0.05), but there was negative correlation between H3 lysine 4 methylation of RASSF1A gene promoter and DNA methylation (r = -0.837, P < 0.05) and no correlation between H3 lysine 9 acetylation and DNA methylation (r = -0.383, P > 0.05).
CONCLUSIONSLaryngeal tumor suppressor gene RASSF1A promoter methylation is a key factor down-regulating the gene expression, and histone modifications also plays an important role in tumor development.
