DNA methylation and histone modification relate to RASSF1A gene deletion in laryngeal carcinoma tissues
10.3760/cma.j.issn.1673-0860.2011.04.011
- VernacularTitle:喉癌组织中RASSF1A基因的表达缺失与DNA甲基化和组蛋白修饰的关系
- Author:
Jing YANG
1
;
Wen-Yue JI
;
Ya-Rong QU
;
Li-Xia HE
;
Xu-Dong ZHAO
;
Ming-Zhu JIN
Author Information
1. 中国医科大学附属盛京医院
- Keywords:
Laryngeal neoplasms;
Tumor suppressor proteins;
DNA methylation;
Histones
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2011;46(4):308-312
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between RASSF1A gene expression and DNA methylation or histone modification in laryngeal carcinoma tissues. Methods Chromatin immunoprecipitation( ChIP), methylation specific polymerase chain reaction(MSP) and realtime quantitative reverse transcription polymerase chain reaction (realtime RT-PCR) were used to analyze RASSF1 A gene promoter region histone H3 lysine 9 methylation, H3 lysine 4 methylation, H3 lysine 9 acetylation, DNA methylation, and RASSF1A gene expression in laryngeal carcinoma tissue of 50 cases. Results DNA methylation rate of gene RASSF1A was 62% in 50 cases of laryngeal carcinoma, but no DNA methylation was found in normal control group, with a significant difference (x2 = 15. 381, P ＜ 0. 05 ). DNA methylation had no correlation with age, gender, differentiation degree, T stage, pathological type and lymph node metastasis ( P ＞ 0. 05 ). The effection of DNA methylation group was more than unmethylation group to expression of gene RASSF1A( t = -3. 108,P ＜ 0. 0l ). There was positive correlation between RASSF1A deletion and gene hypermethylation or between H3 lysine 9 methylation of RASSF1 A gene promoter and DNA methylation in laryngeal carcinoma tissue( r = 0. 816 ,P ＜ 0. 05 ), but there was negative correlation between H3 lysine 4 methylation of RASSF1 A gene promoter and DNA methylation ( r = - 0. 837, P ＜ 0. 05 ) and no correlation between H3 lysine 9 acetylation and DNA methylation ( r = - 0. 383, P ＞ 0. 05 ). Conclusions Laryngeal tumor suppressor gene RASSF1 A promoter methylation is a key factor down-regulating the gene expression, and histone modifications also plays an important role in tumor development.
