Biomechanical study on decellularized laryngeal scaffold in dogs
10.3760/cma.j.issn.1673-0860.2011.04.015
- VernacularTitle:犬去细胞喉支架的生物力学研究
- Author:
Li XU
1
;
Peng-Cheng CUI
;
Zhi-Feng CHEN
;
Rui-Na MA
Author Information
1. 第四军医大学唐都医院
- Keywords:
Larynex;
Stents;
Thyroid cartilage;
Tensile strength;
Compressive strength;
Dogs
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2011;46(4):331-335
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the biomechanical characteristics of the decellularized laryngeal scaffold. Methods Ten Chinese adult dogs were randomly divided into two groups: perfusion group (n=5) and control group ( n = 5). The acellular larynx scaffold was obtained from dogs through cranial thyroid arteries perfusion with detergents. Comparative examinations were performed by the macroscopic view,histological view ( hematoxylin and eosin stain, Alcian blue stain and Masson stain), scanning electron microscope (SEM) and biomechanical properties between perfusion group and control group. Results Macroscopic view showed that the decellularized laryngeal scaffold appeared pale asphyxia.HE stain indicated that there were little acellular traces of muscle and mucosa. Alcian blue stain, Masson stain and scanning electron microscope(SEM)suggested that there were no obvious changes about lycosaminoglycan and collagen. The compressive modulus of thyroid cartilage was ( 1.06 ±0. 07) MPa ((-x)±s) in experimental groups and (1.15±0.11) MPa in control group , showing no significant difference (t=1.424,P＞0.05),neither in compressive modulus of annular cartilage(1.68±0.11)MPa in experimental groups and (1.67±0. 09)MPa in control group (t = 0.185, P＞0.05). The tensile strength of thyroid cartilage between experimental (5.74±0.88) MPa and control groups (6.18±1.33) MPa did not have the statistical significance(t =0. 627, P ＞0. 05 ). Conclusion These results indicate that perfusion method can construct a perfect biomechanical acellular larynx scaffold which could be a better selection for laryngeal reconstruction with tissue engineering method.
