A guinea pigs model of otitis media with effusion caused by reversible Eustachian tube obstruction

VernacularTitle:可逆性咽鼓管阻塞建立豚鼠急性分泌性中耳炎模型
Author: Xin-Yan CUI ¹ ; Chen-Jie YU ; Feng CHEN ; Xia GAO
Author Information

1. 南京医科大学
Keywords: Otitis media with effusion; Disease models,animal; Eustachian tube; Guinea pigs
From: Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2011;46(5):413-416
CountryChina
Language:Chinese
Abstract: Objective To develop a animal model for acute otitis media with effusion (OME). Methods In 22 guinea pigs, the left nasal orifice of Eustachian tube was approached via a transpalatal incision and obstructed with polyvinyl acetal material. Right ears were set as the control. Then all the ears were evaluated by otomicroscopy every day. Seven, 14 and 21 days after the intervention, six guinea pigs were killed for histologic study. Results Of the 22 guinea pigs included in this study, 20 ears (90. 9% ) were found to have effusion 3-7 days after the operation, two cases were excluded for purulent otorrhea 10 days postoperatively. The epithelium initially developed hyperplasia, and the submucosa showed vascular and lymphatic dilatations with inflammatory cells infiltration. None of the contralateral control ears had evidence of disease by otomicroscopic examination and histologic study. Conclusion This experimental methods provoked reproducible pathologic characteristics similar to those for otitis media with effusion.